ABSTRACT
Objective To observe the dynamic histological changes of condylar cartilage ,and detect expression of estrogen re‐ceptors and collagen typeⅡin the ovariectomized rat ,exploring the relationship between OA and abnormity of shorting estrogen . Methods Histological changes of condylar cartilage of the ovariectomized rat at different ages were observed by HE staining meth‐od .The expression of estrogen receptors and collagen typeⅡwere determined by immunohistochemical method ,and the average per‐centage of positive staining area was measured by image analyzer .Results The lack of estrogen led to the degeneration of condylar cartilage .The expression of ER and ColⅡ were suppressed at low concentration of estrogen ,and these effects were enhanced when the treating time was prolonged .Conclusion The lack of estrogen might lead to the rat′s condylar cartilage lesion ,and the low con‐centration of estrogen could suppress the expression of ER .These results highlight the results that expression of condylar cartilage collagen is associated with estrogen .
ABSTRACT
According to the theory of nucleic acid metabolism, the different select models were designed By means of physical and chemical derived to the production of inosine Bacillus Subtilis JSIM 1019, we have got the mutative strains of auxotroph of different component, the mutative strains lacking of relating enzyme, the mutative strains resisting some metabolic analog in turn Restrain of the production to metabolite has been relieved Two mutative strains of auxotroph xanthine, X 13 and so on were obtained And it also has resistance to 8 azaguanine After the mutative strains had been separated into the single srain respectively, we got X 13 4 the maximum accumulation of adenosine reachs 12 43g/L in substratum cultivated for 72 hours at 36℃